Fig. 6. Altered morphology and behavior of EGL cells transduced by dominant
negative Pax6 virus. (A) Viral constructs used for expressing Pax6-EnR and
EnR. Infected cells express a marker gene, alkaline phosphatase (AP), through
an IRES sequence. HD, homeodomain; PD, paired domain. (B,D) Cells infected
with EnR viruses appear in the IGL and display a granule cell morphology with
small, round soma, parallel fibers and claw-like dendrites. (C,E) The EGL
cells infected with Pax6-EnR viruses fail to migrate and cluster around the
injection site. Neither parallel fibers nor mature granule cells in the IGL
are observed. Scale bars: 40 µm in B,C; 20 mm in D,E. (F) The percentages
of infected cells found in each layer.
