Fig. 1. Nkx2.5 and Gata-4 are expressed in the splanchnic mesoderm of the
pharyngeal floor. (A) Whole-mount (stage 14) chick embryos after in situ
hybridization for Nkx2.5. (B) Diagram illustrating the distal cardiac outflow
tract as it is seen in A. The broken line indicates the plane of section seen
in C,E. (C) Nkx2.5 mRNA can be seen in the caudal wall of the distal cardiac
outflow tract (oft) and in the mesenchyme dorsal and caudal to the cardiac
outflow tract (pharynx, p). (D,E) In situ hybridization showing expression of
GATA-4 in the region where Nkx2.5 is expressed (arrow in D). Scale bars: 100
µm in A,C,E; 50 µm in D.
