Fig. 3. Presence of CGH-1 in adult hermaphrodite gonads. (A-K) Extruded gonads from one-day old wild type (A-I) and cgh-1(RNAi) (J and K) hermaphrodites were stained with affinity purified rat CGH-1 antibody (A,D,G,J), an antibody against the constitutive P granule component PGL-1 (B,E,H) (Kawasaki et al., 1998), and the DNA dye DAPI (K), then examined by confocal (A-I) or fluorescence microscopy (J,K). C,F and I are merged images of CGH-1 and PGL-1 staining. (A-C) Individual section through the gonad center that highlights CGH-1 staining in the core region. The distal tip is at top left. No CGH-1 staining was visible in the spermatheca, which is to the bottom left and not shown. Nuclei that stain with DAPI are located in the center of the P granule rings (not shown). (D-F) Detail of the surface of a different gonad, immediately distal to the loop area. (G-I) A section through the center of oocytes. Arrowheads in G indicate some of the CGH-1 staining that colocalizes with PGL-1. Examination of multiple focal planes revealed that in oocytes many PGL-1 particles were larger than the corresponding CGH-1 staining area, but also that in general a discrete focus of CGH-1 staining was co-localized with each P granule (not shown). For example, the very large P granule located to the lower right of the star in H was co-localized with a CGH-1 particle in an adjacent focal plane. Only diffuse background CGH-1 staining is detected in cgh-1(RNAi) gonads (J).
