Fig. 4. CGH-1 expression in adult male gonads. Extruded male wild type (A-D) and cgh-1(RNAi) (E,F) gonads were stained with anti-CGH-1 (A and E), anti-PGL-1 (B), DAPI (D,F), and examined by fluorescence microscopy. A merged image of A, B and D is shown in C. In each panel, an enlargement of the boxed area is shown. CGH-1 staining is less prominent in male gonads than in hermaphrodites, necessitating longer exposure times. In addition to the granular staining detailed in A, these exposures reveal a diffuse pattern, which may represent background. The granular CGH-1 staining is not observed in cgh-1 (RNAi) male gonads (E). d, distal and p, proximal.
