Fig. 5. Presence of CGH-1 in the embryo. Embryos of the 2-cell (A-D), late 4-cell (E-H), approximately 50-cell (I-L) and approximately 100-cell (M-P) stages were stained with anti-CGH-1 (A,E,I,M), anti-PGL-1 (B,F,J,N) and DAPI (D,H,L,P) and examined by fluorescence microscopy. C, G, K and O are merged images. Examination of four-cell embryos by confocal microscopy indicated that, in general, each PGL-1 particle co-localized with a CGH-1 particle (not shown).

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