Fig. 6. Effects of cgh-1 RNAi on oogenesis and spermatogenesis. (A and B) Nomarski DIC views of gonads within 1-day-old wild type (A) and cgh-1(RNAi) (B) hermaphrodites. d, distal; l, loop; s, sperm; o, oocytes; do, degraded oocytes and e, embryo. (C-J) Expression of germline proteins in gonads extruded from 1-day-old wild type (C,E,G,I) and cgh-1(RNAi) (D,F,H,J) hermaphrodites. Gonads were stained with antibodies to the NOTCH homolog GLP-1 (Crittenden et al., 1994; C-D), the meiotic protein GLD-1 (Jones et al., 1996; E-F), the yolk receptor RME-2 (Grant and Hirsh, 1999; G-H), and the P granule component PGL-1 (I-J). d, distal; p, proximal. (K, L) Sperm that were prepared from wild-type and cgh-1(RNAi) gonads were induced with pronase to visualize their pseudopods. Boxes surround a detailed view.
