Fig. 2. In situ hybridization to detect the expression of repulsive cues in the optic chiasma and that of their receptors in the optic nerves. (A) Netrin 1 expression in a frontal section of a postnatal day 1 (P1) rat brain. The ependymal layer of the third ventricle and the lateral edges of the optic chiasma were netrin 1 mRNA-positive. (B) In a horizontal section of the optic chiasma, a netrin 1 signal was found in the anterior pole and lateral edge of the optic chiasma. (C) A Sema3a signal was also found in the anterior pole and lateral edges of the optic chiasma in a horizontal section. Arrows indicate the significant accumulation of signals. (D) Many cell nuclei in the optic nerve were positive for Unc5h1 mRNA. The inset shows the sensing probe signal. (E) Many cell nuclei were also positive for Nrp mRNA. The inset shows the sensing probe signal. (F) Results of RT-PCR using mRNA obtained from a newborn rat optic nerve. Lane 1, Unc5h1; lane 2, molecular marker 0X174 HacIII digested; lane 3, Nrp. CH, optic chiasma; OP, optic nerve. The calibration bar in A-C is 100 µm and that in D-E is 10 µm.
