Fig. 1. Purification of novel galactose-binding proteins from P. misakiensis. (A) Anion exchange chromatography of crude extracts. The third peak (arrowhead) was further fractionated. (B) Gel filtration HPLC. The highest peak (arrowhead) has a retention time of about 20 minutes. (C) SDS-PAGE of the highest peak after gel filtration HPLC. (D) P18 and P15 after a second anion exchange chromatography step. (E) Affinity chromatography of P18 and P15, on immobilized galactose. After washing with the binding buffer containing 1 mM CaCl2, the column was eluted with 5 mM EDTA. (F) SDS-PAGE of each fraction after affinity chromatography.
