Fig. 5. Effects of recombinant proteins on cell growth and differentiation of the atrial epithelium in culture, 5 days after inoculation in the serum-supplemented medium. (A-C) Phase contrast microscopy. Scale bar, 50 µm. (D-G) Immunocytochemistry. Cells were suspended in the medium by pipetting and mounted on poly-L-lysine-coated cover slips. Cells, except those in F, were stained with the monoclonal antibody recognizing specifically alkaline phosphatase of the atrial epithelium. In F, the gastric epithelium-specific antibody was used. (A,D) rTC14-2 (3 nmol/ml). (B,E,F) rTC14-3 (3 nmol/ml). (C,G) rTC14-3 (3 nmol/ml) + 50 µM galactose. Scale bar, 25 µm.
