Fig. 4. Histological analysis of LjPax6 expression. (A-G) Transverse sections of a stage 26 embryo except for the whole-mount embryo in A, in which the levels of the sections (B-G) are shown by arrows. Box indicates the enlarged portion in H. It is clear from A, that LjPax6 is expressed in the nasohypophysial plate (arrow). (B-G) High levels of LjPax6 transcripts are found in the dorsal telencephalon (arrow in B), the diencephalon (arrows in C,D), the optic stalk (arrowheads in C-E), the eye (arrowhead in F) and the dorsal oral ectoderm (arrow in G), whereas no transcripts are seen in the midbrain (arrow in F). (H) LjPax6 expression in the oral ectoderm. LjPax6 expression is seen in the dorsal oral ectoderm (DOE), the anterior velum ectoderm (AVE) and the posteriodorsal velum ectoderm (PDVE).
