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Fig. 6. (A) Xfz3 specifically induces neural crest. RT-PCR analysis of Xslug expression is shown for animal caps expressing chordin (1 ng) alone or with Xfz2 (0.1 ng, 1 ng), Xfz3 (0.1 ng, 1 ng), Xfz7 (0.1 ng, 1 ng), Xfz8 (0.1 ng, 1 ng) and Xwnt1 (0.1 ng). (B) Dendrogram analysis of Drosophila, mouse and Xenopus frizzleds. (C) Nxfz3, but not Nxfz2 or Nxfz7, inhibits Xwnt1 induction of Xslug. RT-PCR analysis of Xslug expression in animal caps expressing chordin (1 ng) alone, Xwnt1 and Nxfz3, 2 or 7 (0.1 and 1.0 ng).





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