Notch signaling is required for arterial-venous differentiation during embryonic vascular development
Nathan D. Lawson1, Nico Scheer2, Van N. Pham1, Cheol-Hee Kim1, Ajay B. Chitnis1, Jose A. Campos-Ortega2 and Brant M. Weinstein1,*
1Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD, 20892, USA
2Institut für Entwicklungsbiologie, Universität zu Köln, 50923 Cologne, Germany
*Author for correspondence (e-mail: bw96w@nih.gov)
Movie 1
Circulation in a 2 dpf wild-type sibling zebrafish embryo. Anterior is towards the left, dorsal is upwards. The first part of the movie shows the anterior of the entire embryo. The next part shows a close-up of the mid-trunk. Robust circulation through the dorsal aorta (DA) and posterior cardinal vein (PCV) can be seen, as well as blood flow exiting the DA and entering PCV via dorsoventrally aligned intersegmental vessels. No blood flow directly between the DA and PCV is observed in this or any other wild-type embryo.
Movie 2
Circulation in a 2 dpf mindbomb mutant zebrafish embryo. Anterior is towards the left, dorsal is upwards. The first two sequences show lower magnifications, the last sequence shows a high-magnification videomicrograph of the mid-trunk. The heart is functioning and trunk circulation is present; however, blood can be seen flowing directly from the dorsal aorta (DA) to the posterior cardinal vein (PCV) through a prominent arterial-venous shunt.
