Fig. 2. Stacking bar charts of representative cumulative cohort experiments set up from the SCG and SG of E12 to E16 embryos (SCG at E12, E14 and E16, and SG at E13 and E15). The cells were grown in either defined medium alone (control cultures) or medium supplemented with 10 ng/ml artemin. An initial cohort comprising all the neurones in a 12x12 mm grid in the centre of each dish was identified 6 hours after plating. The number of surviving neurones in this cohort was monitored at 6 hourly intervals and is expressed as a percentage of the initial cohort at 6 hours (black bars). New neurones that were generated in the grid between 6 and 12 hours, 12 and 18 hours, 18 and 24 hours, 24 and 30 hours, 30 and 36 hours are expressed as a percentage of the initial cohort, and their survival was likewise subsequently monitored at 6 hourly intervals after their appearance (patterned and white bars).
