
Fig. 3. (A) Graph of the number of new neurones generated between 6 and 36 hours after plating in dissociated cultures of sympathetic ganglia of E12 to E16 embryos in defined medium alone (control) or medium supplemented with 10 ng/ml artemin. Bar charts of the number of BrdU-labelled neurones in dissociated E12 SCG (B) and E13 (C) stellate ganglion cultures grown either in defined medium alone (control) or medium supplemented with 10 ng/ml artemin. In E12 cultures, BrdU was added 2 hours after plating and the cultures were fixed and stained 16 hours after plating. In E13 cultures, BrdU was added 24 hours after plating and the cultures were fixed and stained 12 hours later. The means and standard errors are shown (n=3-6 for each condition).