Fig. 1. Effects of microinjection of As-T2/En^R mRNA on the expression of muscle-specific actin gene (HrMA4) and myosin heavy chain gene (HrMHC), assessed by whole-mount in situ hybridization. (A-F) Expression of HrMA4 and (G-Q) HrMHC. (A-C,G-I,O-Q) Embryos around the 110-cell stage, (D-F,J-L) at the early tailbud stage and (M,N) at the 64-cell stage. (A,D,G,J,M) Control embryos; (B,E,H,K,N) embryos developed from eggs injected with 0.2 µg/µl As-T2/En^R mRNA and (C,F,I,L) those injected with 0.01 µg/µl As-T2/En^R mRNA. (O-Q) Co-injection of 0.2 µg/µl As-T2/En^R mRNA with (O) 0.05 µg/µl As-T2, (P) 0.2 µg/µl As-T2 or (Q) 0.1 µg/µl HrBra mRNA. Scale bar: 100 µm.

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