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Fig. 6. Requirement of the distal and proximal T-binding motifs of As-T2 for its upregulation assessed by the reporter gene expression. (A,B) p(-351)({Delta}Td)As-T2/lacZ was injected solely (A) or with As-T2 mRNA (B). (C,D) p(-351)({Delta}Tp)As-T2/lacZ injected solely (C) or with As-T2 mRNA (D). Injected eggs were allowed to develop to the 110-cell stage, and then cleavage was arrested for about 12 hours before detection of the reporter gene expression.





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