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Fig. 8. Efficiency of T-binding motifs in expression of HrMA4 (A-D) and HrMHC (E-H). (A-D) The reporter gene expression in embryos at the 110-cell stage injected with p(-216)HrMA4/lacZ (A), p(-216)HrMA4/lacZ with As-T2 mRNA (B), p(-216)({Delta}T)HrMA4/lacZ (C) and p(-216)({Delta}T)HrMA4/lacZ with As-T2 mRNA (D). (E-H) The reporter gene expression in embryos at the 110-cell stage injected with p(-132)HrMHC/lacZ (E), p(-132)HrMHC/lacZ with As-T2 mRNA (F), p(-132)({Delta}T)HrMHC/lacZ (G) and p(-132)({Delta}T)HrMHC/lacZ with As-T2 mRNA (H). Injected eggs were allowed to develop to the 110-cell stage, and then cleavage was arrested for about 12 hours before detection of the reporter gene expression.





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