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Fig. 2. Expression of GFR{alpha}1 and Ret proteins declines in ciliary ganglion neurons after target innervation. (A) Western blot characterization of anti-GFR{alpha}1, GFR{alpha}2 and Ret antibodies used in the present study. The GFR{alpha}2 antiserum generated in this study recognizes an approx. 55 kDa protein, equivalent to the estimated size of GFR{alpha}2, in E9 ciliary ganglion neurons. The anti-GFR{alpha}1 antibody recognizes two protein species of approx. 55 and 65 kDa, whereas the anti-Ret antibody recognizes a single protein of 170 kDa in ciliary ganglion neurons. (B) Western blot analysis for GFR{alpha}1, GFR{alpha}2, Ret, CNTFR{alpha} and gp130 in ciliary ganglion lysates (left) or whole brain lysates (right) at different embryonic ages.





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