
Fig. 2. HP-1 immunostaining pattern in interphase nuclei from blastoderm embryos. (A) Female nuclei. Immunostaining for HP-1 antibody (left) and staining for DNA (middle). The merged image (right) shows the punctate distribution of the HP-1 immunolabeling (green) over the DAPI-stained nuclei (red). (B,C) Male nuclei. Immunostaining for HP-1 antibody (left) and staining for DNA (middle). The merged image (right) shows the co-localization of the HP-1 immunolabeling (green) with the chromocenter in DAPI-stained nuclei (red). Note in B the two nuclei in the middle with the chromocenter split into two masses. In C, a prometaphase cell (top right) is also shown. Scale bar: 10 µm.