Fig. 1. In situ analysis of cell death E9-E9.5. (A) Acridine Orange staining of pyknotic cells in unfixed embryos (19 somites). Conventional fluorescence microscopy (inserts) at low power reveal similar sized and stained embryos, despite differences in folding and angles of view (left panel, wild type=Igf2+m/+p; right panel Igf2+m/-p). Confocal microscopy and maximum projection of the same embryos (18x
5µm sections at 20 µm intervals, large images) reveal the distribution of multiple areas of focal labelling above background (see text). (B) TUNEL assay of 7 µm cryosection (wild type=Igf2+m/+p embryo (upper) and littermate Igf2+m/-p (lower)). A complex distribution of labelling was observed in both sections. Scale bars: 500 µm in A; 100 µm in B.
