Fig. 4. DNA content and cell cycle parameters of embryo suspensions. (A,B) Representative distribution of DNA content at E9.25 (aged by cell number) of wild-type (A) and Igf2+m/-p (B) litter-matched embryos. Gates: Sub-G1=a, G1=2xb, S=c-(b+d) and G2=2xd. (C-F) Quantification of Sub G1(C), G1(D), S (E) and G2 (F) from DNA profiles of litter-matched embryos, as in A,B aged by wild-type embryos at E8.4±0.2 (n=10), E8.8±0.2 (n=26), E9.25±0.25 (n=18), E9.75±0.25 (n=24), E10.15±0.15 (n=22), E10.75±0.25 (n=6) and E11.52±0.25 (n=18). The differences were significant (*) only at E9.25 for sub-G1 (C, P=0.003). E9.75 S-phase (E, P=0.001) and E9.75 G2 (F, P=0.008) (Student’s t-test, 95% confidence interval). For D-F, percentages were of total number of viable cells (excluding subG1). Error bars indicate s.e.m.
