Fig. 2. Overexpression of (A) dominant negative dn Xgsk3ß in wild-type embryos induced small eyes or loss of eyes as evaluated at 2 dpf. This phenotype was not induced either by overexpression of (B) full-length fl Xgsk3ß or by (C) frame shift fs Xgsk3ß. Numbers of embryos per treatment are indicated (top of graph) pooled from (A) 4, (B) 7 and (C) 4 independent experiments. (D-F) Phenotypes at 3 dpf or (G) at 24 hpf (lateral view, rostral to the left, dorsal up) of (D) wildtype, and upon overexpression of (E) dn Xgsk3ß that induced small eyes (left) or loss of eyes and forebrain (right), or (F,G) full-length fl Xgsk3ß that induced deletion of the ventral forebrain and partial eye fusion. (H-J) emx1 expression, at approx. 30 hpf, in (H) wild type is restricted to the telencephalon (arrow), but overexpression of dn Xgsk3ß (I,J) resulted in (I) eye reduction accompanied by an expanded emx1 domain in a few cases, and (J) in loss of eyes accompanied by normal emx1 expression in the majority of cases. (K,L) fgf8 expression in a squash preparation (dorsal view) at approx. 30 hpf in (K) wild type, and (L) an embryo overexpressing dn Xgsk3ß, showing small eyes accompanied by loss of optic stalk labelling (right panel). 1, MHB; 2, optic stalks; 4, dorsal diencephalon; 5, retina. mRNAs (320 pg/1 nl) were injected into one-cell stage embryos.
