Fig. 4. (A,B) Frequencies of phenotypes at 2 dpf of embryos from in-crosses of heterozygous mbl fish upon overexpression of (A) dominant negative dn Xgsk3ß that induced loss of eyes in approx. 80% of embryos, and (B) full-length fl Xgsk3ß, which rescued eyes of mbl-/- embryos. Numbers of embryos pooled from (A) 4 and (B) 5 independent experiments are indicated at the top of the graph; n.i., non-injected. (C-E) Eye phenotypes (lateral view, rostral to the left, dorsal up) at 3 dpf of (C) wild-type embryo from an eyeless clutch, (D) mbl embryo from an eyeless clutch, (E) overexpression of full-length fl Xgsk3ß rescued eyes in mbl. (F-H) flh expression at the tailbud stage. (G) in mbl, anterolateral borders of the neural plate (anterodorsal view) ectopically express flh compared to (F) wild type (arrowheads). (H) Overexpression of full-length fl Xgsk3ß reduced ectopic expression of flh (arrowhead, ectopic flh in mbl). mRNA (320 pg/1nl) was injected into one-cell stage embryos.
