Fig. 5. emx1 expression at approx. 30 hpf is restricted to the telencephalon in wild-type embryos (A). In mbl embryos with small eyes (B) and (C) eyeless, this expression is anteroventrally expanded. (D) Overexpression of full-length fl Xgsk3ß in mbl embryos rescued eyes but did not normalize the mutant emx1 domain. (E-H) fgf8 expression at approx. 30 hpf in (E) wild-type embryos and (F) eyeless mbl embryos that lost the expression in retinae and optic stalks and still showed weak expression in facial ectoderm (FEC) and dorsal diencephalon and a normal expression at the MHB. (G,H) Overexpression of full-length fl Xgsk3ß in mbl embryos rescued expression in one optic stalk and one eye (G) and (H) both eyes and fully rescued wild-type fgf8 expression. (A-H, lateral view, rostral to the left; G,H lower panels in a frontal view.) 1, MHB; 2, optic stalks; 3, FEC; 4, dorsal diencephalon; 5, retina. mRNAs (320 pg/1nl) were injected in 1-cell embryos.
