Fig. 4. IFE-1, IFE-3 and IFE-5 are enriched in the germline. m7GTP affinity-purified proteins derived from 1.5 mg of total protein from wild-type (N2) or germline-deficient (glp-4) worms were resolved by SDS-PAGE on 6% (A) and 12% (B) gels. (A) The gel on the left was stained with silver nitrate, the gel on the right was immunoblotted using anti-PGL-1 antiserum. (B) The gels were immunoblotted using antibodies to isoform-specific C-terminal peptides for each of the IFE proteins. Both wild-type and glp-4 worms were grown at 25°C, the restrictive temperature for germline development in the glp-4 strain.
