Fig. 6. The YSL is not required to maintain the endodermal identity of ECB and activated cells during gastrulation. (A) Diagram of the experimental procedure. At the blastula stage, activated cells (Tar* + GFP) or ECB were transplanted to the animal pole of host embryos. Animal caps containing the grafted cells (B) were immediately removed, cultured and fixed at the end of gastrulation (90% epiboly). (C,D) sox17 expression was revealed by in situ hybridisation (blue), and activated cells were identified by detection of nuclear ß-galactosidase (brown, D). (D) Inset shows a close-up view of the region containing transplanted cells.
