Fig. 5. Neurogenesis of Sox10+/– mutant cells in high density cultures. Undifferentiated p75-positive cells isolated from DRG of wild-type and Sox10+/– mutant E13 embryos were incubated at high density in differentiation medium. Wild-type cells produced O4-positive glia (A) while de novo neurogenesis did not occur. Arrowhead in C indicates a NF-positive sensory neuron present at time of isolation. Only few O4-positive glia were generated in Sox10+/– mutant cultures (arrow in E). Many mutant cells formed aggregates of NF-positive neuronal cells (arrow in G). Insets in G,H show a neuronal cluster at higher magnification. After prolonged incubation, neuronal cells acquired features of fully differentiated neurons expressing peripherin (Per) (arrow in I).
