Fig. 6. Expression analysis of Artn mRNA in developing rat embryos. Radioactive in situ hybridization for Artn mRNA on parasagittal sections of E12.5 (A), 14.5 (B,C) and 16.5 (D-F) rat embryos. High levels of Artn mRNA are detected in the dorsal aorta (A,B, white arrows), the superior mesenteric, vertebral and celiac arteries (B,D, arrowheads). At E14.5, the vertebral artery also expresses high levels of Artn mRNA (C). Note that Artn expression in the dorsal aorta is undetectable at E16.5 (D, arrow). By contrast, persistent high expression is detected in small branches of the mesenteric arteries (arrows in dark- (E) and bright- (F) field photographs) at E16.5. Scale bar: 500 µm in A,C,E,F; 250 µm in B; 1100 µm in D.
