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Fig. 3. Forelimb and pectoral girdle defects in Alx mutants. Alizarin Red (bone) and Alcian Blue (cartilage) stained forelimbs and pectoral girdles. Genotypes: (A,D) Alx3+/-/Alx4+/+; (B,E) Alx3+/+/Alx4lst-J/lst-J; (C,F) Alx3-/-/Alx4lst-J/lst-J. Arrowheads indicate the clavicle and arrows indicate (normal position of) deltoid crest. Asterisk marks extra digit in B,C. (D-F) Dissected clavicles; double arrows and L-M indicate lateral-medial orientation of the clavicles.
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