Fig. 9. Study of phenotype by analysis of expression patterns of marker genes in serial sections. Probes used are specified to the right. (A,E,G-I,M-O) Control embryos (either Alx3 heterozygotes or wild type); (B,C,F,J-L,P-R) Alx3^-/-Alx4^lst-J/lst-J. (A-F) Serial sections of two E10.5 embryos and expression of ectodermal markers, where D is a scheme to indicate approximate plane of sections in the embryos used in this experiment. (G-R) Nearby serial sections of two control and two double mutant embryos and expression of two mesenchymal markers. (G,J,M,N) E10.5 embryos; (H,I,K,L,N,O,Q,R) Rather advanced E11.5 embryos. Orientation of sections of this series of embryos is depicted in lower row of panels. Arrows (in A-C) indicate Shh expression in ectoderm of the medial nasal processes. Abbreviations: BA, branchial (mandibular) arch; FB, forebrain; FNP frontonasal process; HB, hindbrain; HE, heart; Man, max, mandibular and maxillary process, respectively, of first branchial arch; MNP, medial nasal process; MP, maxillary process; NC, nasal cavity; NS, nasal septum; O, otocyst; Ps, palatal shelf (part of maxilla); To, tongue.

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