Fig. 10. Synapse localization is disrupted in unc-119(ed3) mutants. (A) Ventral view of a newly hatched wild-type L1 larvae expressing GFP-tagged synaptobrevin in DD neurons. Presynaptic varicosities are indicated by an arrow. (B) Ventral view of a newly hatched unc-119(ed3) L1 larvae expressing GFP-tagged synaptobrevin in DD neurons (arrow). (C) There were no synaptic varicosities visible in the dorsal nerve cord of wild-type L1 larvae (arrow). (D) Presynaptic varicosities were seen in the dorsal nerve cord of unc-119(ed3) mutants (arrow). (E) Dorsal nerve cord varicosities were observed in 52% of unc-119(ed3) DD3 axons (wild-type worms: 0% n=21 (black); unc-119: n=23 (gray)). Scale bars (A-D) 5 µm.
