Fig. 4. Effects of Foxd3^Myc electroporation at stage 28. (A,B,D,E) Foxd3^Myc-expressing cells have migrated extensively and can be seen encircling neurons in the dorsal root ganglia (A,D,E, arrows). Foxd3^Myc+ cells do not co-express Brn3.0 (A) or Phox2a (B), which mark sensory and sympathetic ganglia, respectively. Ectopic Foxd3^Myc+ cells do not express markers of postmitotic neurons, TuJ1 (D) and NeuN (E). (C) Ectopic expression of the Schwann cell marker, P0, in the neural tube induced by Foxd3 misexpression. Foxd3^Myc+/P0⁺ cells are marked by arrows. (F) Electroporation with a control CS2-Myc expression vector. Note that Myc-tag expressing cells enter the dorsal root ganglia and differentiate as sensory neurons (arrow). drg, dorsal root ganglion; fp, floor plate; sc, spinal cord; sym, sympathetic ganglia.

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