Fig. 3. Inhibition of Meis function results in reduced krox20 expression in both wild-type and lzr mutant embryos, while overexpression of meis1.1WT partially rescues the lzr mutant phenotype. Each panel is oriented with anterior towards the left. (A-D) krox20 expression (in r3 and r5) in wild-type embryos at 8-10 somites injected with mRNAs shown on the left. Note the decrease in krox20 expression caused by Meis1.1
C and Meis1.1N323D in C,D, as compared with wild type in A. (E-H) krox20 expression in lzr- embryos injected as shown. Note the increase in krox20 expression caused by expressing Meis1.1WT, by comparing E,F. Genotypes of embryos were determined subsequent to photography using PCR-mediated dHPLC (Pöpperl et al., 2000).
