Fig. 3. pou2 expression in wild-type, spg and noi mutant embryos. Expression of pou2 in wild-type (A-E), spgm793 (F-H), spgm216 (I), and noitu29a (J) mutant embryos. MHB expression of pax2.1 (red; D,I) or eng2 (orange; E,J) relative to that of pou2 (blue). Dorsal (B-E,G-J) or lateral (A,F) views of whole-mount embryos with animal pole/anterior at the top; (D,E,I,J) dorsal views of the neural plate at higher magnification. Embryonic genotypes are indicated in the top right-hand corner of each image; developmental stages are indicated in the bottom right-hand corner. hb, hindbrain; mb, midbrain; %, % epiboly; tb, tailbud stage; som, somites (stage).
