Fig. 8. An AP gradient of nuclear ß-catenin in the presumptive neural plate during gastrulation. (A) Mid-gastrula embryos were cut sagittally and left and right halves were analysed by in situ hybridization for Gbx2 expression and by immunostaining for ß-catenin, respectively. Eight consecutive regions of the presumptive neuroectoderm of immunostained halves were selected from posterior to anterior positions, according to the gastrula fate map. Corresponding windows are shown in B for one representative embryo. Note prominent nuclear staining in windows 1 and 2, and absence of nuclear staining in 7 and 8. The AP axis is indicated (P
A) and the approximate position of the Gbx2 expression domain is given in relation to the windows. Data in C represent mean normalized densities of the nuclear regions of all cells within a window.
