Fig. 1. EGFRs mediate chemotaxis after viral transduction of explants. (A) How the explants were dissected and manipulated to alter the orientation of infected cells relative to ligand. Dorsolateral cortex was used in all experiments except L, where dorsolateral and medial cortex was used. In all micrographs, the ventricular surface is down, whether explants were grown VZ up or down, and the relative location of ligand is indicated. At least three explants were analyzed per condition. Similar distributions of cells were noted in triplicates. (B) E12.5 mouse, control virus, grown VZ down, no ligand; (C) E12.5 mouse, control virus, VZ down, TGF
10 ng/ml; (D) E12.5 mouse, control virus, VZ up, no ligand; (E) E12.5 mouse, control virus, VZ up, HB-EGF 10 ng/ml; (F) E12.5 mouse, EGFR virus, VZ down, no ligand; (G) E12.5 mouse, EGFR virus, VZ down, HB-EGF 1 ng/ml; (H) E12.5 mouse, EGFR virus, VZ up, HB-EGF 1 ng/ml; (I) E10.5 mouse, EGFR virus, VZ up, HB-EGF 10 ng/ml; (J) E12 rat, EGFR virus, VZ down no ligand; (K) E12 rat, EGFR virus, VZ down, TGF 0.1 ng/ml; (L) E12 rat, EGFR virus, VZ down, TGF 1 ng/ml (arrow indicates midline between hemispheres); and (M) E12 rat, EGFR virus, VZ up, TGF 10 ng/ml.
