Fig. 7. Infection at E10.5 results in region-specific changes in migration. (A) Micrograph of control-infected cells 6 days after injection at E10.5. Note clusters of cells in cortical plate (CP). (B) Micrograph of EGFR-infected cells 6 days after infection at E10.5. Note cells in subplate/white matter (SP/WM) in lateral and ventrolateral cortex, but not dorsomedial cortex, where they remained in proliferative zones (VZ, ventricular zone). Insets: higher magnification images of cells in dorsomedial (left) and ventrolateral (right) cortex. In ventrolateral cortex, EGFR-infected cells were found in the lateral cortical stream (C,D), migrating toward the piriform cortex (P). (C) Brightfield micrograph of X-gal labeled cells, (D) DAPI. (E,F) Control-infected cells were also found in the lateral cortical stream (E, brightfield; F, DAPI). (G,H) EGFR-infected cells (arrows) in the lateral cortical stream were found among cells that express high endogenous EGFRs. G was stained with an antibody that recognizes both endogenous and virally transduced EGFRs; in H, the EGFR-infected cells could be distinguished by expression of ß-galactosidase.
