Fig. 2. Abr and Bcr are expressed in the postnatal cerebellum. (A) Bcr and Abr protein levels were examined in the cerebellum at P1-15, in adult wild-type cerebellum (a1), in adult Abr-/-;Bcr-/- cerebellum (a2) and in primary cerebellar astrocytes isolated at P0. In total cerebellar extracts, two forms (96 kDa and 91 kDa) of Abr are present, whereas cultured astrocytes express only the smaller 91 kDa form. Null mutants lacked both 96 and 91 kDa forms but the antiserum used aspecifically reacts with proteins in that size range. (B-E) In situ hybridization of wild-type midsagittal sections of the cerebellum at the age of 8 days using Abr (B,D;, left panel) and Bcr (C,E, right panel) probes. (B,C) Anti-sense probes. (D,E) Sense probes used as a negative control. Arrows in B,C point to intensely stained Purkinje cells. Scale bars: 150 µm.
