Fig. 5. Aberrant ventral GT histogenesis induced by perturbing Shh signaling. Anti-Shh antibody addition induced ventral dysmorphogenesis (A, control section; B, 5E1 treated section which lacks midline urethral plate). Anti-Shh antibody mediated inhibition of mesenchymal Fgf10 gene expression in the ventral GT (coronal sections (C,D); C, control section; D, 5E1 treated section; arrowheads indicate the expression). GT phenotype of Gli2 mutants. Gli2-/- mice (16 dpc) display drastic abnormalities in the ventral GT formation phenocopying the Ab-treated specimen (E,F, coronal sections; E, wild type; F, Gli2-/- mutant). Aberrant ventral shape is marked by a line (B,F). Scale bars: 62.5 µm in A-D; 31.25 µm in E,F.
