Fig. 5. Localisation of AtRPS5A mRNA by in situ hybridisation. Five-day-old wild-type seedlings were hybridised with an AtRPS5A-specific antisense (A-F) or sense (G) probe. Untreated seedlings (A-E,G) as well as NAA-treated seedlings (F) were used. Expression is detected in young leaf primordia (A). (B) A close-up of the apical shoot region of a seedling. Expression is confined to the leaf primordia (lp1 and lp2) and the shoot apical meristem (sam). A significant signal is detected in the vasculature of cotyledons (C), and the primary root tip (D). (E) A close-up of a root apical region. A strong signal is present in all dividing cells, but not in the quiescent center (qc). NAA-induced lateral root primordia stain strongly for the AtRPS5A signal (F). (G) The absence of a signal in a sense-probe control on an untreated seedling root.
