Fig. 2. Response of dpp-lacZ to gro loss of function in the wing. groE48 clones are marked by loss of GFP (green A,C) or Myc epitope (green, B) and ß-galactosidase is shown in red (red channel shown separately in A'-C'). A subset of gro- clones in A (some clones are outlined in blue) express dpp ectopically. This effect is non-autonomous, as neighbouring wild-type cells are also induced to express dpp (arrows). Ectopic dpp-lacZ is never seen anterior to its normal stripe in hhE23 groE48 clones (C), suggesting that its ectopic expression is mediated by hh activity. The few ß-galactosidase-positive cells in the anterior clones in C are within the normal dpp expression stripe. dpp-lacZ is autonomously derepressed in anterior clones posterior to the normal expression stripe (B), where it would be normally downregulated by En. This can happen in gro- (A,B) as well as in hh- gro- (C) clones – marked by arrowheads. All disks are oriented with anterior towards the left and dorsal upwards. The straight blue line indicates the position of the AP boundary.
