Fig. 3. CNS and motor axon phenotypes produced by RNAi. Dissected late stage 16 embryos stained with mAb 1D4. Column 1 (A,E,I), wild-type. Column 2 (B,F,J), embryos injected with injection buffer. Column 3 (C,G,K), embryos injected with Dlar dsRNA. Column 4 (D,H,L), embryos injected with Ptp52F dsRNA. Row 1 (A-D), the CNS (anterior up); note the 3 straight longitudinal bundles on each side of the midline in A-C. Arrow in D indicates a break in the outer 1D4 bundle; arrowhead indicates abnormal fasciculation of the middle and outer bundles. Row 2 (E-H), ISNb (anterior towards the left); note the 3 characteristic synaptic branches in E,F,H; muscles are numbered in E. Arrow in G, bypass ISNb; arrowhead, ISN; asterisk, SNa. Row 3 (I-L), SNa; note the characteristic bifurcation in I-K, indicated by arrow in (I); muscle 12 is also labeled. Asterisk in L, approximate position of missing bifurcation.
