Fig. 5. ced-10 is expressed broadly. Anterior, left; dorsal, top. Scale bars: 5 µm in A; 2.5 µm in B,C; 5 µm in D,E. (A) A mid-L1-stage larva showing GFP::CED-10 expression in all cells examined, including the pharynx, axons of the nerve ring, and neurons along the ventral nerve cord (vnc), where the GABAergic D-class motoneurons reside. GFP::CED-10 localized to the plasma membrane in vnc neuron cell bodies. (B) A different focal plane of the animal in A showing expression of GFP::CED-10 in the hypodermis. The hypodermal seam cells (V cells) are noted with arrows. GFP::CED-10 accumulated at the plasma membrane in these cells. (C) An L2 larva with plasma membrane accumulation of GFP::CED-10 in vnc motoneuron cell bodies. Intestinal expression is also shown. (D) GFP::CED-10 accumulation at the plasma membrane of the CAN neuron cell body in an adult animal. The fused hypodermal seam syncytium also showed plasma membrane-localized GFP::CED-10. (E) An L4 larva with expression of GFP::CED-10 in the posterior distal tip cell of the developing gonad.
