Fig. 3. Lmd is a C₂H₂-type of Zn-finger protein with homology to Gli family members. (A) Diagram of the predicted Lmd protein. Checked and black boxes denote the conserved Zn-finger domain and putative phosphorylation sites, respectively. Position of the mutation in lmd¹ (nucleotide change from C to T, converting the Gln residue at position 127 to a nonsense residue) is indicated. (B) Sequence comparison of the Zn-finger domain of Lmd and representatives of the Gli superfamily was done using the Clustal W program: Lmd (Accession Number, AY032609); mouse Gli3 (Accession Number, Q61602; mouse Gli1 (Accession Number, BAA85004); human Gli2 (Accession Number, P10070); Drosophila Ci (Accession Number, A38926); C. elegans Tra-1 (Accession Number, P34708); mouse Zic4 (Accession Number, Q61467); ascidian Macho-1 (Accession Number, BAB19958). The Cys and His residues of each finger are highlighted in black. Amino acid residues that are identical or similar in at least 50% of the aligned sequences at a particular position are boxed in dark or light gray, respectively. (C) Phylogenetic tree of Lmd and representatives of the Gli superfamily, based upon Zn-finger domains shown in B. Programs Clustal X and TREEVIEW (Page, 1996) were used to generate the tree that displays the possible way in which the protein sequences may have evolved.

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