Fig. 4. lmd gene expression in somatic mesodermal cells is severely affected in wg and abolished in N mutant embryos. Embryos were hybridized with a lmd RNA probe (A-D). (A) Dorsal view of late stage 11 embryo with expression in patches of visceral mesoderm. (B) Lateral view of late stage 12 embryos with expression in both somatic and visceral mesodermal layers. (C,D) Stage 13 embryos show decreasing expression in somatic mesodermal cells. Expression is no longer detectable in visceral mesodermal cells. (E-G) Embryos were hybridized to a lmd RNA probe (green) and stained with anti-Eve antibody (red), followed by confocal microscopy. When compared with control embryo (E), wg^cx4 mutant embryo (F) shows a dramatic decrease in lmd expression in somatic mesodermal cells in dorsolateral and lateral regions while ventrally located cells (sm; arrow) are not strongly affected. Visceral mesoderm (vm; arrow) is expanded in mutant embryo. In N⁵⁴¹⁹ mutant embryo (G), lmd expression is abolished in all somatic mesodermal cells. Visceral mesoderm is reduced in mutant embryo.

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