Fig. 4. Complex formation with Chip is required to stabilize Ap protein. (A) Wing imaginal disc with homozygous Chipe5.5 mutant clones. Clones were marked by the absence of ß-gal (right). Ap levels decreased in mutant clones, compared with the surrounding tissue (left panel, arrows). Ap levels were higher in homozygous wild-type twin spots, which contain two copies of the Chip gene, than in the tissue heterozygous for Chip (arrowheads). (B) ptcGal4 uas-EGFP; uas-Chip
LID wing disc. ChipLID overexpression was visualized by co-expression of GFP (left panel). Ap expression was reduced (anti-Ap shown in white, center). ap-lacZ transcript levels reflected by anti-ß-Gal did not differ (right). (C) dppGal4 uas-EGFP; uas-ChAp wing disc. ChAp and GFP were co-expressed (left; GFP shown in green). Endogenous Ap protein was reduced to background levels in these cells (right panel, arrow). Note the anti-Ap antibody does not recognize ChAp.
