Fig. 7. Overexpression of dLAp-flag causes an ap gain-of-function phenotype. (A) Cuticle preparation of an ap^Gal4; uas-dLAp-flag wing. Overexpression of dLAp-flag caused extra vein tissue between veins 4 and 5 and other vein defects. (B) ap^Gal4; uas-dLAp-flag fly showing the abnormal wing posture and upward curvature of the wing caused by the reduced size of the D compartment. (C) Serrate protein expression (green) in a disc overexpressing uas-ap in the ap^Gal4domain. The pattern of Serrate staining is the same as in wild-type discs and shows elevated expression along veins 3 and 4 and on both sides of the DV boundary. (D) Expression of uas-dLAp-flag caused overexpression of Serrate in the D compartment. Ectopic Serrate staining can be seen in intervein regions (e.g. arrow). Note the reduced size of the D compartment. (E) dLMO and Wg protein expression in an ap^Gal4; uas-dLMO; uas-ap third instar wing disc. Anti-dLMO (green) and Anti-Wg (blue). Note the small wing pouch (arrow) and the absence of Wg expression at the interface between D (green) and V (not green) cells. (F) dLMO and Wg protein expression in an ap^Gal4; uas-dLMO; uas-dLAp third instar wing disc. Note that Wg expression is restored along the DV boundary and growth of the wing pouch is restored.

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