Fig. 3. Distribution of OOC-5 in embryos. Confocal micrographs of wild-type embryos (A-O) double labeled with anti-OOC-5 (A,D,G,J,M) and anti-tubulin antibodies (B,E,H,K,N); merged images are shown in C,F,I,L,O, where the overlap in signal appears yellow. Confocal images of ooc-5(it145) mutant embryos double labeled with anti-OOC-5 (P,R) and anti-tubulin (Q,S) antibodies. (A-C) One-cell embryo at prophase; (D-F) one-cell anaphase; (G-I) two-cell interphase; (J-L) two-cell embryo in which the AB cell (left) is in anaphase and P1 is in metaphase. (M-O) Four-cell embryo in which the AB cells are dividing (top left cells, spindle poles only visible), EMS is in prophase and P2 (right most cell) is in interphase. (P-Q) One-cell anaphase ooc-5 mutant embryo. (R,S) Two-cell interphase ooc-5 mutant embryo. The stage of the cell cycle was determined by DAPI staining (not shown) in addition to microtubule staining for all embryos. Posterior is towards the right in this and all figures. Scale bar: 10 µm.
