Fig. 5. OOC-5 localization depends on OOC-3 in embryos, but OOC-3 localization does not require OOC-5. (A-I) Confocal micrographs of ooc-5 (it145) embryos double labeled with anti-OOC-3 (A,D,G) and anti-HDEL antibodies (B,E,H). Merged images show the extent of co-localization (C,F,I). (A-C) One-cell prophase embryo with one pronucleus visible; the other pronucleus is out of the plane of focus. (D-F) One-cell embryo in anaphase. (G-I) Two-cell embryo in interphase. (J-R) Confocal micrographs of ooc-3 (mn241) embryos double labeled with anti-OOC-5 (J,M,P) and anti-HDEL antibodies (K,N,Q). Merged images show the extent of co-localization (L,O,R). (J-L) One-cell prophase embryo with one pronucleus visible. (M-O) One-cell embryo in anaphase. (P-R) Two-cell interphase. All confocal images were obtained using the same settings as for the wild-type embryos in Fig. 4. Scale bar: 10 µm.
