Fig. 3. Tube gradient formation is regulated at the level of the Tube/Pelle interaction. (A) dl¹ embryo stained with antibodies to Tube detected with a Cy3-conjugated secondary antibody. Surface view of the anterior pole, lateral aspect. (B) Wild-type embryo stained with antibodies to Toll detected with a Cy3-conjugated secondary antibody. Surface view of the ventral aspect. (C,D) pll⁰⁷⁸ embryos stained with antibodies to Toll as above; composite images, surface view of the lateral aspect. In each image, an arrow indicates the approximate position of the ventral midline.

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